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61.
西藏鱼类增殖放流初报 总被引:4,自引:0,他引:4
西藏土著鱼类生长缓慢、性成熟晚并对环境变化高度敏感。近年来,受过度捕捞、水利工程建设等人类活动的影响,西藏渔业资源面临越来越大的压力。开展增殖放流活动是恢复当地渔业资源和生态平衡的重要手段。本文介绍了西藏鱼类增殖放流概况、苗种来源、放流实施、效果评估情况,分析了目前存在的基础研究不足、苗种来源不稳定、缺乏技术规范、效果评估有待加强等问题。提出了加强增殖放流基础设施建设和基础理论研究、建立西藏鱼类增殖放流与效果评估技术规范、重视放流后的管理与评估工作、加强专业技术人才队伍建设的建议。 相似文献
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Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety 下载免费PDF全文
Zheng‐Jun Pan Chuan‐Kun Zhu Hui Wang Guo‐Liang Chang Huai‐Yu Ding Xiao‐Gang Qiang Xiang‐Sheng Yu 《Aquaculture Research》2017,48(11):5659-5665
The bagrid catfish, Pseudobagrus ussuriensis, exhibits significant sexual dimorphism in growth rate and body size with males growing faster than females. Therefore, an all‐male culture can dramatically increase the output and profitability of fishery products. According to the monosex breeding route, super‐male individuals’ acquirement by XY male sex reversal and artificial gynogenesis is the key step. An effective protocol to induce meiotic gynogenesis using homologous sperms has been developed in this study. The most effective UV irradiation for sperm genetic inactivation was found to be at a distance of 20 cm with 66 μW/cm2 light intensity for 25 min. Optimal treatment for cold shock was 5 min post‐fertilization at 0‐4°C for 30 min, which gave the best survival rate of 13.65 ± 2.87%. The sex ratio in the meiotic gynogens showed a significant female‐biased deviation (p < .05); thirty meiogynogens and their parents were further analysed using a male‐specific AFLP marker, of which only the male parent produced a male‐specific DNA band of 412 bp. These results indicated the female homogametic XX/XY sex determination system in P. ussuriensis. The optimization of a protocol for the successful induction of meiogynogenesis in the bagrid catfish lays the basis for all‐male production and is useful in ascertaining the genetic sex determination system in this promising aquaculture species. 相似文献
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Ting Zou Qiao Xiao Wenjie Li Tao Luo Guoqiang Yuan Zhiyuan He Mingxing Liu Qiao Li Peizhou Xu Jun Zhu Yueyang Liang Qiming Deng Shiquan Wang Aiping Zheng Lingxia Wang Ping Li Shuangcheng Li 《Rice》2017,10(1):53
Background
Male fertility is crucial for rice yield, and the improvement of rice yield requires hybrid production that depends on male sterile lines. Although recent studies have revealed several important genes in male reproductive development, our understanding of the mechanisms of rice pollen development remains unclear.Results
We identified a rice mutant oslap6 with complete male sterile phenotype caused by defects in pollen exine formation. By using the MutMap method, we found that a single nucleotide polymorphism (SNP) variation located in the second exon of OsLAP6/OsPKS1 was responsible for the mutant phenotype. OsLAP6/OsPKS1 is an orthologous gene of Arabidopsis PKSA/LAP6, which functions in sporopollenin metabolism. Several other loss-of-function mutants of OsLAP6/OsPKS1 generated by the CRISPR/Cas9 genomic editing tool also exhibited the same phenotype of male sterility. Our cellular analysis suggested that OsLAP6/OsPKS1 might regulate pollen exine formation by affecting bacula elongation. Expression examination indicated that OsLAP6/OsPKS1 is specifically expressed in tapetum, and its product is localized to the endoplasmic reticulum (ER). Protein sequence analysis indicated that OsLAP6/OsPKS1 is conserved in land plants.Conclusions
OsLAP6/OsPKS1 is a critical molecular switch for rice male fertility by participating in a conserved sporopollenin precursor biosynthetic pathway in land plants. Manipulation of OsLAP6/OsPKS1 has potential for application in hybrid rice breeding.67.
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Root rot symptoms were observed in fields of alfalfa in Chifeng city, Inner Mongolia, China in 2016. Disease incidences of seven alfalfa varieties planted in 2014 ranged from 56% to 95%, while incidence of Gongnong No. 1 planted in 2016 was 8%, 31% and 76% in 2016, 2017 and 2018, respectively. Paraphoma isolates were consistently recovered from black necrotic root tissues of diseased plants with a frequency of 77.1%. Based on morphological characters and phylogenetic analysis of rDNA internal transcribed spacer (ITS), elongation factor 1-α (EF1-α) and β-tubulin (TUB), this fungus was identified as Paraphoma radicina. Glasshouse pathogenicity experiments showed that P. radicina significantly reduced above- and below-ground biomass of alfalfa plants 2 months after inoculation. Paraphoma radicina infected 70% of the plants inoculated with a root dip in conidia, and these symptoms were consistent with the symptoms in the field. Paraphoma radicina was successfully reisolated from disease roots of the inoculated alfalfa plants. This is the first report of P. radicina as the causal agent of alfalfa root rot in China. 相似文献
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对福建、江西、浙江、湖南、湖北、贵州、重庆、四川、海南、云南及广西11省(自治区、直辖市)70个县(区、市)的楠属(Phoebe)树种种质资源进行详细调查,记录楠属种质资源分布、群落现状、木材利用及人工造林情况,对种质资源进行收集保存,并详细介绍主要树种的特征差异。调查发现,中国楠属树种资源主要分布在长江流域及以南地区,其中紫楠(P.sheareri)、闽楠(P.bournei)及白楠(P.neurantha)分布范围最广;综合比较生长速度和适应性,广西适宜推广种植的楠属树种有闽楠、桢楠(P.zhennan)及崖楠(P.yaiensis)。 相似文献